Атанас Павлов – Atanas Pavlov

PLANT IN VITRO TECHNOLOGY FOR FOODS AND FOOD ADDITIVES: CURRENT STATUS, SPECULATIONS AND FUTURE PROSPECTS

Atanas Pavlov

1University of Food Technologies, 26 Maritza Blbd., 4002 Plovdiv, Bulgaria
2Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria
at_pavlov@yahoo.com

From time immemorial, humans have been highly dependent on plants as sources of proteins, carbohydrates and fats. Furthermore, the volume and range of phytochemicals used by modern society (inter alia as drugs, nutrients, cosmetic additives and biopesticides) are continuously expanding. On the other hand, the critical global challenges facing humanity are achieving sustainable development in the context of global climate change, limited access to fresh water, limited food supply and growing energy needs. These high demands are driving efforts to develop new ways to produce plant-derived metabolites. Plant in vitro techniques, in which plant cells, tissue and organs are cultivated under controlled conditions offer effective tools for sustainable supply of phyto-ingredients with reduced energy, carbon and water footprints. The advantages of this technology over the conventional agricultural production are: 1) independence of geographical and seasonal variations and various environmental factors; 2) insurance of the continuous supply of products with uniform quality and yield; 3) possibility to produce novel compounds that are not normally found in parent plants; 4) efficiency in downstream recovery and product; 5) reduced energy, carbon and water footprints; etc.

The lecture will be focused on integrated approaches for bioactive substances production in different plant in vitro systems with application in food products. Current status, speculations and future prospects as well as challenges of the commercialization of the products of plant cell and tissue cultures will be outlined. The specific technological requirements for the final product formulations will be discussed. Finally, some examples of products developed in our laboratory will be presented as an eco-friendly alternative methods for sustainable production of plant-derived additives with application in foods.


Атанас Павлов

Биография: Роден през 1967 г. в Пловдив, България; 1992 г. – магистърска степен по биотехнологии в Университет по хранителни технологии (УХТ) – Пловдив; 1998 г. – „доктор“ по биотехнологии в Института по микробиология при Българската академия на науките (БАН);  2003 г. – 2005 г. – постдокторант в Институт по хранителни технологии и биопроцесорно инженерство към Техническия университет в гр. Дрезден, Германия. Професионално развитие: 1998 г. –  учен в Института по микробиология при БАН; 2005 г. – доцент в същия институт. 2009 г. –  „доктор на науките“; 2010 г. – професор в Института по микробиология при БАН. От 2011 г. е и преподавател в УХТ – Пловдив. През 2014 г. става член-кореспондент към БАН, а през 2018 г. и на Саксонската академия на науките. От 2021 г. е академик към БАН. Научни интереси: биологично активни вещества от растителни in vitro системи; биопроцесорно инженерство на растителни in vitro системи. Библиография:  5 монографии; 2 учебника; 13 глави от книги; 190 публикации, 105 от които реферирани в WoS/Scopus; 115 доклада на научни конференции. Педагогическа работа: 12 курса лекции за български и чуждестранни университети; 10 защитили докторанти. Други: експерт към немското федерално министерство на образованието и изследванията; Експерт към полския национален научен фонд; Co-Editor на Engineering in Life Science.

Atanas Pavlov

Biography: Born in 1967 in Plovdiv, Bulgaria; 1992 – Master’s degree in Biotechnology at the University of Food Technology (UFT) – Plovdiv; 1998 – PhD in Biotechnology at the Institute of Microbiology at the Bulgarian Academy of Sciences (BAS); 2003 – 2005 – post-doc at the Institute of Food Technology and Bioprocess Engineering at the Technical University of Dresden, Germany. Professional development: Since 1998 a scientist at the Institute of Microbiology at the BAS, and since 2005 Associate Professor at the same institute. In 2009 received the degree of “Doctor of Science”, and since 2010 is a Professor at the Institute of Microbiology at the BAS. Since 2011 he is a lecturer at UFT – Plovdiv. In 2014 he became a Corresponding member of the BAS, and in 2018 of the Saxon Academy of Sciences. Since 2021 he is an Academician at the BAS. Research interests: biologically active substances from plant in vitro systems; bioprocess engineering of in vitro plant systems. Bibliography: 5 monographs; 2 textbooks; 13 chapters from books; 190 publications, 105 of which are referenced in WoS/Scopus; 115 reports at scientific conferences. Pedagogical work: 12 courses of lectures for Bulgarian and foreign universities; 10 defended PhD students. Others: expert at the German Federal Ministry of Education and Research; Expert at the Polish National Science Foundation; Co-Editor of Engineering in Life Science, Germany.

ANABOLICS – MUSCLES IN A PILL?

Oldrich Lapcik

Department of Chemistry of Natural Compounds, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technicka 5, 16628 Praha 6, Czech Republic
lapciko@vscht.cz

The relationship between masculinity and musculature has been revealed since ancient times. Testicular extracts with strengthening effects are mentioned in the literature from ancient Rome (Gaius Plinius Secundus, 23-79) to almost the present (Ch-E. Brown-Séquard, 1817-1894).

The discovery of steroid hormones and a synthetic approach to them in the 1930s opened up new possibilities (Butenadt, Ruzicka). Orally active testosterone analogues (17α-methyltestosterone, Ruzicka 1935) have been included in the repertoire of medicinal steroids since 1935. Shortly after confirmation of their therapeutic potential (replacement of endocrine function of the testicles, reconvalescence acceleration after injuries) anabolic steroids began to gain a ground in strength sports.

Abuse of high doses has led to numerous side effects, including the deaths of athletes. Anabolics have been on the IOC Prohibited Substances List since 1975, and the World Anti-Doping Association (WADA) Prohibited Substances and Methods List is updated annually (https://www.wada-ama.org/sites/default/files/2022-01/2022list_final_en_0.pdf). Since 2008, non-steroidal selective androgen receptor modulators (SARMs) have been added to the list.

Anabolics are at the forefront of statistics on doping cases in competitive sports – 47% of positively tested samples in WADA laboratories in 2020. A serious phenomenon today is the spread of anabolics in recreational sports (bodybuilding, fitness), which is mainly mediated by online stores. Dosage regimens go far beyond those that would be appropriate for therapeutic use; drugs of dubious origin and composition are often used, including those that have already been ruled out by medicine for their side effects.


Oldřich Lapčík

CV: Narozen 1960 ve Zlíně, Česká republika. V roce 1989 absolvent magisterského studia (obor: biochemie) na Přírodovědecké fakultě University Karlovy v Praze (PřF UK Praha). V roce 1998 absolvent doktorského studia (obor: biochemie) na PřF UK Praha. Profesionální praxe: Endokrinologický ústav Praha (1989-2001), Vysoká škola chemicko- technologická (VŠCHT Praha; 2002-dosud) Od roku 2009 profesor na Ústavu chemie přírodních látek, Fakulta potravinářské a biochemické technologie, VŠCHT Praha. Výzkumné stáže v Německu, Finsku a Švédsku. Vedoucí řady bakalářských, magisterských a doktorských prací. Výzkum: Koordinátor nebo partner 8 národních projektů. Bibliografie: Autor nebo spoluautor 5 patentů, 2 kapitol v monografiích, 102 původních vědeckých článků a 80 odborně-popularizačních článků. Ostatní odborná činnost: Člen národních i mezinárodních vědeckých společností, člen vědeckých rad řady institucí, člen redakční rady odborně popularizačního časopisu Vesmír.

Oldřich Lapčík

CV: 1960 born in Zlín, Czech Republic. 1989 graduated in Biochemistry at Faculty of Science, Charles University Prague, Czech Republic. 1998 Doctorate in Biochemistry at Faculty of Science, Charles University Prague, Czech Republic. Working activities: Institute of Endocrinology Prague  (1989-2001), University of Chemistry and Technology Prague (UCT Prague; 2002-up to now). Since 2009, full professor at the Department of Chemistry of Natural Compounds, Faculty of Food and Biochemical Technology, UCT Prague. Several research stays in Germany, Finland, Sweden. Mentoring numerous bachelor, master and doctoral thesis. Research: Coordinator or partner in 8 national projects. Bibliography: Author or co-author of 5 patents, 2 chapters in monographs, 102 scientific papers, many contributions presented on international symposiums, 80 works popularizing science. Additionally: Member of several advisory boards, member of the editorial board of the science popularization journal Vesmír.

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Blaženka Kos

NEXT-GENERATION PROBIOTICS AND PREBIOTICS – AN EFFICIENT STRATEGY FOR BALANCING THE HUMAN MICROBIOTA

Blaženka Kos, Jagoda Šušković, Jasna Novak, Andreja Leboš Pavunc, Martina Banić, Katarina Butorac, Nina Čuljak

Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
bkos@pbf.hr

Research of the human gut microbiome has confirmed the presence of phylogenetically diverse bacterial species with health-promoting effects and potential of faecal microbiota transplantation as long-term engraftment of the patients gut microbiota. Microbiota present on mucosal and skin surfaces also impacting the interactions along the gut-liver-brain axis. According to the results of our research, selected and functionally characterized autochthonous strains of lactic acid bacteria (LAB), isolated either from the breast milk microbiota or artisanal fermented food, have a huge potential as a next-generation of probiotics [1]. They produce specific macromolecules such as surface S-layer proteins (Slps), bacteriocins, exopolysaccharides (EPS) and proteolytic enzymes, which are associated with their probiotic activities. Slps and EPS contribute to the stress-resistant properties of the producer cells during biotechnological production by freeze-drying as well as during gastrointestinal transit, and adherence in the colon, with potential immunomodulatory activity. Additionally, EPS are also prebiotic substrates for the gut microbiota – producers of short-chain fatty acids and lactate, metabolites with antibacterial and immunomodulatory activity, energy supply for the enterocytes, and the modulation of cholesterol and lipid metabolism. Bacteriocins, Slps and EPS promote competitive exclusion of pathogens, whereas bacteriocins and biopeptides-released upon the proteolytic activity, display antimicrobial activities. Biopeptides have also shown anti-inflammatory, ACE-inhibitory and antioxidant activities. Selected LAB strains have promising applications, ranging from the design of future functional food, food supplements or live biotherapeutic products. The impact of next-generation probiotics and prebiotics on human microbiota balance will be further defined through an advanced meta-omics approach and data analysis by new bioinformatics algorithms and analysis tools.

Acknowledgments

This work was supported by the Croatian Science Foundation – projects IP-2019-04-2237; IP-2014-09-7009.

Reference

[1]  K. Butorac, M. Banić, J. Novak, A. Leboš Pavunc, K. Uroić, K. Durgo, N. Oršolić, M. Kukolj, S. Radović, S. Scalabrin, J. Žučko, A. Starčević, J. Šušković, B. Kos, Microb. Cell Fact. 19 (2020) 106.


Blaženka Kos

CV: Rođena je 1968. godine u Zagrebu, Hrvatska. Akademske stupnjeve: dipl. ing. (1992.), magistar znanosti (1996.) i doktor biotehničkih znanosti, pod mentorstvom prof. dr. sc. Jagode Šušković (2001.), stekla je na Prehrambeno-biotehnološkom fakultetu (PBF-u) Sveučilišta u Zagrebu. Radno iskustvo: od 1992. je zaposlena u Laboratoriju za tehnologiju antibiotika, enzima, probiotika i starter kultura, Zavoda za biokemijsko inženjerstvo na PBF-u gdje radi kao redoviti profesor u trajnom zvanju. Pedagoške aktivnosti: Od 1992. godine podučava studente preddiplomskih, diplomskih i poslijediplomskih studija na PBF-u, kao i internacionalnog preddiplomskog studija „Blue Economy and Growth“ (EU-CONEXUS Minor programmes), diplomskog studija “Molecular and Industrial Biotechnology” (Sveučilište Insubria)  i „Prehrambeno inženjerstvo“ (Sveučilište u Mostaru). Bila je mentor 6 doktorskih radova. Istraživačke aktivnosti: Bila je suradnik i voditelj na projektima financiranim iz nacionalnih i internacionalnih fondova, od industrije, kao i u okviru Znanstvenog centra izvrsnosti BioProCro. Bibliografija: Publicirani znanstveni radovi su joj citirani u Web of Science više od 1600 puta, a h-indeks iznosi 21. Dodatno: Suradnica je Akademije tehničkih znanosti Hrvatske. Više je puta nagrađivana za svoja istraživanja mehanizma djelovanja, modulacije crijevne mikrobiote domaćina, biotehnološke proizvodnje i primjene probiotika i funkcionalnih starter kultura.

Blaženka Kos                      

CV: Blaženka Kos was born in 1968, in Zagreb, Croatia. She graduated and obtained engineer title in 1992, received her MSc degree in 1996 and PhD degree under the mentorship of prof. Jagoda Šušković in 2001 at Faculty of Food Technology and Biotechnology (FFTB), University of Zagreb. Work experience: Since 1992 she is employed in Laboratory of antibiotic, enzyme, probiotic and starter culture technologies, Department of biochemical engineering, at FFTB, where she currently works as a full professor having a tenure. Pedagogical activity: Since 1992 she is involved in education of undergraduate, graduate and doctoral students at FFTB, as well as on the international undergraduate study „Blue Economy and Growth“ (EU-CONEXUS Minor programmes), on the graduate study „Molecular and Industrial Biotechnology“ (University of Insubria) and “Food Engineering” (University of Mostar). She has mentored 6 PhD Theses. Research activities: As a researcher or leader, she has actively participated in projects funded through various national and international funding agencies and industries, and through Centre of Excellence – BioProCro. Bibliography: The scientific merit of her publication output is corroborated by more than 1600 citations with the current WoS measured h-index of 21. Additionally: She is associate of the Croatian Academy of Engineering. She was several times awarded for her achievements in research of mode of action, modulation of the intestinal microbiota of the host, biotechnological production and application of probiotics and functional starter cultures.

IMPORTANCE OF AMINO ACIDS AND BIOGENIC AMINES IN FOOD QUALITY AND NUTRITION

Livia Simon Sarkadi

Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Somlói út 14-16.,1118, Budapest, Hungary
simonne.sarkadi.livia@uni-mate.hu

The re-recognition of the important role of health-conscious nutrition has brought revolutionary changes in both food science and food production. A greater knowledge of the nutritional content of foods is needed to understand fully the food/health interactions, which could facilitate more efficient production of foods tailored to promotion of human health. Based on their essential role in the body, amino acids and their derivatives, biogenic amines, can be of great importance for food quality and nutrition. It is well known that 20 αL-amino acids are involved in the structure of proteins in genetically encoded form. The nutritional value of a protein depends on how well the essential amino acid composition merits the requirements of the human body. In addition to protein-building amino acids, free amino acids content and their changes during processing and storage play important role in food quality, and taste of food and may have beneficial effects on human health. Other components that influence food quality and health are biogenic amines. In food biogenic amines are mainly formed as a result of microbial decarboxylation of amino acids. Consumption of food containing high amounts of biogenic amines is responsible for many pseudo-allergic food related reactions and increased levels of biogenic amines in food are indicators of its microbiological quality. There are significant differences in the nutritional quality of the two major types of food, of plant and animal origin. However, the assessment must also take into account the new concept of sustainable food production and consumption. Research on amino acids and biogenic amines dates back decades, but remains an excellent area for research due to new perspectives and challenges.


Simonné Sarkadi Livia

CV: 1980- Okleveles Vegyészmérnök, Budapesti Műszaki Egyetem (BME); 1986 Dr.Techn. Biokémia, BME; 1991, Kémiai Tudományok Kandidátusa (CSc), Magyar Tudományos Akadémia (MTA); 1999 Dr. Habil., BME; 2010 MTA Doktora, Kémiai Tudományok (DSc). Munkahelyek/Oktatói tevékenység: BME Biokémiai és Élelmiszertechnológiai Tanszék/Alkalmazott Biokémiai és Élelmiszertudományi Tanszék (tanársegéd-egyetemi tanár, 1980-2012); Tanszékvezető egyetemi tanár Budapesti Corvinus Egyetem Élelmiszerkémiai és Táplálkozástudományi Tanszék (2012-2016)/Szent István Egyetem (SZIE 2016-2021)/Magyar Agrár- és Élettudományi Egyetem (MATE 2021-); Rektorhelyettes, SZIE (2018-2020); Élelmiszertudományi Doktori Iskolavezető SZIE/MATE (2019-). Kutatómunka: az aminosavak, biogén aminok és a fehérjék élelmiszerminőséggel és -biztonsággal való összefüggésének feltárása, valamint az abiotikus növényi stressz biokémiai vizsgálata. Tudománymetria: 388 tudományos cikk, idézettség 2360, H-index: 23. Egyebek: Elnök, Magyar Kémikusok Egyesülete (2011-); Elnök, MTA Élelmiszertudományi Tudományos Bizottság (2019-); Elnök, EuChemS Élelmiszerkémiai Divizió (2009-2014); Igazgatótanács tag, EuCheMS (2014-2021); Igazgatótanács tag, ECTN (2019-); Magyarország képviselője a GHI hálózatban. Megkapta az IUPAC 2015 Distinguished Women in Chemistry/ Chemical Engineering Awards díjat.

Livia Simon Sarkadi

CV: 1980 Dipl.-Ing in Chemical Engineering, Budapest Technical University (BUTE); 1986 Dr.Techn. in Biochemistry, BUTE; 1991 Candidate of Chemical Sciences (CSc), Hungarian Academy of Sciences (HAS); 1999 Dr. Habil., BUTE; 2010 Doctor of Science (DSc) in Chemical Sciences, HAS. Working/pedagogical activity: Department of Biochemistry and Food Technology/Department of Applied Biochemistry and Food Science, BUTE (assistant professor-full professor, 1980-2012); Head of Department of Food Chemistry and Nutrition, Corvinus University of Budapest (2012-2016)/Szent István University (SZIE 2016-2021)/Hungarian University of Agriculture and Life Sciences (MATE 2021-); vice rector of SZIE (2018-2020); head of Doctoral School of Food Science, SZIE/MATE (2019-). Research area: food quality and food safety, with a particular emphasis on amino acids, biogenic amines and proteins, and the biochemical aspects of abiotic plant stress. Bibliography: 388 scientific articles, citations 2360, H-index: 23. Additionally: President of the Hungarian Chemical Society (2011-); Chair of the Food Science of HAS (2019-); Chair of the Food Chemistry Division of EuChemS (2009-2014), Member of the EuCheMS Executive Board (2014-2021); Member of Administrative Council of the European Chemistry Thematic Network Association (ECTN); Ambassador of Hungary in Global Harmonisation Initiative network (GHI). She received the IUPAC 2015 Distinguished Women in Chemistry/ Chemical Engineering Awards.

HEALTH PROMOTING PROPERTIES OF MUSHROOMS

Marina Sokovic

Institute for Biological research “Sinisa Stankovic”, National Institute of Republic of Serbia, University of Belgrade, Bulevard Stefana Despota 142, 11 000 Belgrade, Serbia
mris@ibiss.bg.ac.rs

Mushrooms are untapped resource of species with both nutritive and medicinal potential, undervalued and underexplored until recent history, but now they have been increasingly utilized in pharmaceutical and food industry, as well in medicine. With reevaluated concept of using food in prophylaxis of diseases and in medicine, contemporary research has been strongly focused on this area, leading to scientifically supported facts. Since nutritive, therapeutic, and finally curative properties of edible and medicinal mushrooms have been praised in both ethnomedicine and scientific/clinical research, these properties will be presented as to evaluate novel prospects and possible applications of edible and medicinal mushrooms.

Mushrooms have immense potential in food/pharmaceutical industries which they owe to the presence of various compounds, both nutritive and nonnutritive. Aside from having incredibly favorable macronutrient profile, they are important source of highly appreciated polysaccharides, lectins, terpenoids, peptides, proteins etc. These compounds are responsible for as many as 130 biological activities including antineurodegenerative, immunomodulatory, antihypercholesterol, antihyperglycemic potential which are most often highlighted. Furthermore, they have immense potential as prophylactic agents in diseases whose underlying cause is associated with oxidative stress (antioxidant potential), bacterial and fungal infections (antimicrobial potential) and tumor cell development (antitumor activity).

Research in the field of medical mycology is of great importance for fundamental science, pharmaceutical and food industry as well as in medicine, it contributes to new knowledge and creates insight into the underlying natural processes. Having in mind the accumulating knowledge on edible and/or medicinal mushrooms, it is believed that the life expectancy and its quality will be significantly improved at the crossroads of Eastern and Western medicine. Cooperation between different aspects of science and marketing must be carried out in order to transfer the acquired knowledge to the public and encourage the use, cultivation and further studies of mushrooms.


Марина Соковић

Биографија: Рођена у Врању, Србија, 1970. год. Диплoмирала на биолошком факултету, Универзитет у Београду (1995), мaгистрирала (1998), дoктoрирала (2001). Радна активност: нaучни сaвeтник Институтa зa биoлoшкa истaрживaњa „Синишa Стaнкoвић“, рукoвoдилaц тимa микoлoшкe лaбoрaтoриje Oдeљeњa зa биљну физиoлoгиjу. Предаје нa Биoлoшкoм фaкултeту Унивeрзитeтa у Бeoгрaду, „Антифунгална активност секундарних метаболита биљака“. Научна активност: биoлoшка aктивнoст прирoдних прoизвoдa, aнтимикрoбнo дejствo, истрaживањa пaтoгeних гљивa. Библиографија: Објавила 300 рaдoва у мeђунaрoдним чaсoписимa, 140 саопштења, 7700 цитата (х индeкс 40). На листи најцитиранијих научника (2%). Педагошки рад: руководила израдом 3 мaгистaрска рaдa, 7 дoктoрских дисeртaциjа. Додатно: После докторске студије завршила је на Plant Research International, Wageningen University, Холандија, стипендија холандске Владе Од 2018. год. рукводилац српског тима, ЕУ пројекта, Horizon 2020, No. 760891. Гoстујући урeдник у мeђунaрoдним чaсoписaма, рeцeнзeнт у 30. Била је члан Савета Универзитета у Београду. Од 2019. обавља функцију помоћника министра за науку у Министартсву просвете, науке и технолошког развоја РС.

Marina Soković

CV: Born in Vranje, Serbia, 1970. Graduated at Faculty of Biology, University of Belgrade (1995), Master in Sciences (1998), PhD degree in Biology (2001). Work activity: Scientific advisor at Institute of biological research “Sinisa Stanković“, head of the research group in laboratory of Mycology. Lecturer at Faculty of Biology, University of Belgrade, Serbia (Biological activity of natural products). Research work: phytochemistry, biological activity of secondary metabolites, mycology, food safety, pathogenic fungi. Bibliography: published 300 in international SCI journals, cited 7700 times, several chapters in books, participated in 140 conferences, h index 40. She is a highly cited scientist (top 2%) and among 10 the most cited Pedagogical work: Mentor of 3 scientific masters and 7 doctoral theses. Additional: postdoc research at the Plant Research International, Wageningen University (The Netherlands) as a Scholar of the Netherlands Government (2002-2003). Leader of few international Projects, including Horizon 2020-No. 760891. She has been the Associate Editor, Editor-in-Chief, Guest Editor, reviewer in 30 journals. a member of the Council Body of the University of Belgrade (2011-2019). Assistant Minister for Science from 2019. at the Ministry of Education, Science and Technological Development of Republic of Serbia.

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