Jaroslav Havlík – Jaroslav Havlik

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY: A VERSATILE AND ROBUST TOOL FOR FOOD FRAUD DETECTION

Jaroslav Havlik, Anna Mascellani Bergo, Lucie Rysova

Czech University of Life Sciences Prague, Kamycka 129, 165 00, Prague – Suchdol, Czech Republic
havlik@af.czu.cz

High-field nuclear magnetic resonance spectroscopy (NMR) is an important tool for food authentication and fraud detection. The method shows unprecedented robustness, minimum sample preparation and quantitative capability, without relying on a calibration with authentic standards. NMR has shown strengths in the detection of origin and adulteration of wine, honey, fruit and vegetable juices, coffee beverages or milk. The method is gaining even more importance hand in hand with the development of automated algorithms in spectral processing, user-friendly chemometrics- and machine learning tools. The talk aims to show a snapshot of state of the art in the field and present our contribution in NMR wine analysis and cow milk adulteration, including new approaches in data processing.

In wines, NMR detects approx. 30-50 most abundant metabolites of various chemical classes. Based on three thousand NMR spectra of authentic wine samples of Czech origin, regression and classification models have shown high precision in predicting some of the wine properties, such as colour, residual sugar, alcoholic strength, or acid content. Random forest algorithm has shown a good precision in classifying varieties in varietal wines. To a limited extent, location and sensory quality could also be predicted. Practical application of the models in market authenticity control will be shown.

NMR has been successfully used in discriminating summer- and winter milk of small ruminants and the breed of the animal. Moreover, NMR has shown great potential in detecting goat milk adulteration with cheaper cow milk. Peaks of N-acetyl carbohydrates are precise biomarkers of cow milk presence; highly predictive models could be built based on their signals for cow milk presence above 5%.

NMR metabolomics research can massively profit from open data databases and standardized workflows, which will be discussed in concluding remarks.

Acknowledgements

This work was supported by METROFOOD-CZ research infrastructure project (MEYS Grant No: LM2018100), including access to its facilities.


Jaroslav Havlík

CV: Narozen 1978 v Liberci. V roce 2003 absolvent studia na České zemědělské univerzitě v Praze (ČZU), obor zemědělství. Získal PhD v oboru zemědělství se zaměřením na fytochemii. Postdok na University of Glasgow (UK) na oddělení lidské výživy. Profesionální praxe: Docent v oboru zemědělská chemie na katedře Kvality a bezpečnosti potravin, Fakulta agrobiologie, potravinových a přírodních zdrojů, ČZU. Lektor předmětů Chemometrika, Epidemiologie výživy, a Kvalita potravin. Výzkum: Řešitel nebo spoluřešitel projektů zaměřených na analýzu potravin, výživu, kvantitativní nukleární magnetickou rezonanci, chemometriku v různých oblastech biologie a zemědělství, metabolomiku stolice, metabolismus polyfenolů. Bibliografie: Autor nebo spoluautor více než 80 vědeckých článků, přes 50 příspěvků na mezinárodních konferencích, h-index 21.  Ostatní odborná činnost: člen Nutrition Society (UK), Microbiome Society (CZ), člen konsorcia H2020 HEDIMED a člen COST akce UNGAP.

Jaroslav Havlik

CV: Born in Liberec (CZ) in 1978, graduated in agriculture at the Czech University of Life Sciences Prague (CZU) in 2003 and received his PhD in phytochemical investigations of medicinal plants. He completed his postdoctoral fellowship at the University of Glasgow (UK) at the Human nutrition section. Working activities: Associate professor in Food Chemistry at the Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, CZU. Lecturer of Chemometrics, Nutritional Epidemiology, Food Quality. Research: Principal investigator or co-investigator in projects related to food analysis, nutrition, quantitative nuclear magnetic resonance and chemometrics in various fields of biology and agriculture, stool metabolomics, polyphenol metabolism. Bibliography: Author/co-author of more than 80 scientific papers, over 50 contributions on international symposiums, h-index 21. Additionally: Member of Nutrition Society, Microbiome Society (CZ), H2020 HEDIMED project consortium partner, associate of UNGAP COST Action.

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